The Good
Fast and easy to use, high yield of exosomes/extracellular vesicles.
The Bad
Higher cost than similar/simpler PEG buffers for isolation, requires optimization with cell culture media (works best without serum or with exosome depleted serum), non-specific isolation of all extracellular vesicles and non-exosomal particulates/aggregates in culture media.
The Bottom Line
This product produces excellent results for fast, easy, and high yield isolation of exosomes and extracellular vesicles without requirements for special equipment like an ultracentrifuge. The trade off compare to other isolation techniques is that the kit will non-specifically isolate non-exosomal aggregates leftover in the media even after pelleting larger cell debris. This kit is a great place to start when initiating and optimizing new experiments involving exosome and EV isolation.