OriGene
Ern1 Mouse qPCR Primer Pair
MP204187
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We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic Aire expressing cells.
qPCR genotype validation
N/A
This primer set did not work using the manufacturer's protocol.
This primer pair failed to amplify IRE1a DNA from either WT or IRE1a KO mice following the manufacturer's protocol. In addition, it had high background amplification in water only control samples.
Cheap qPCR probe
Did not amplify, would require user optimization to work.
Not a useful reagent for validating IRE1a KO genotypes.