Invitrogen
Dynabeads His-Tag
10103D
Info:Supplier Page
I used these beads to perform coIP in cell lysates. I like these magnetic beads because they do not use an antibody to capture your protein of interest. This removes the possibility of eluting your antibody off of the beads along with your protein of interest. For this, this was an issue because of the cross-reactivity of antibodies with my secondaries in western blotting.
Co-immunoprecipitation
Cell lysates
Make sure your second protein of interest does not have a his tag!!!
In the end, I was unable to use this product because my second protein of interest had an endogenous his tag. But the beads worked beautifully to IP the his tagged protein that I transfected.
N/A
Works great.
Doesn't work if both proteins have a his tag.
I recommend this over traditional coIP.