Easy to Use Antibody for KLRG-1 Staining

Results Summary

I used this antibody to analyze both T cell and NK cell differentiation. This antibody and clone worked well for me and I was able to detect a clear signal for KLRG-1 expressing T cells and NK cells. I used this antibody on a Cytek 5L Aurora in combination with ZombieNIR and the NK cell lineage marker NK1.1 in APC/Fire810 or CD4 APC/Fire810; all these combinations worked well together without a noticeable loss of signal. Accordingly, I am very pleased with the signal that this fluorochrome is able to produce and the ease of use of this antibody. At a titrated optimal concentration of 1:200, it is also fairly economical to use this antibody. Accordingly, we have used it in our lab for multiple years. I would recommend to use this antibody. In the following, you will find my staining protocol in short. Incubation steps were performed at 4°C in the dark. 1.Count and adjust splenocytes to 1.5E6 cells, plate in a 96-well plate. 2.If required, stain cells with viability dye. Wash. 3. Stain with extracellular master mix for 20 min. Wash 2x. 4. Fixate/ Permeabilize with buffer of choice, depending on application. I used ThermoFisher FoxP3 fixation/permeabilization kit for 20 min. Wash with perm/ Wash 2x. 5. Perform intracellular staining for 20 min. Wash 2x. 6. Resuspend in FACS buffer and acquire as soon as possible. Here, data was acquired on a 5L Cytek Aurora or 5L BD LSRFortessa with the appropriate filters.