CD15 Antibody for Flow Cytometry Staining of MDSC

December 14, 2022

Anaesthesiology
LMU Munich
Researcher

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Company:

BioLegend

Product Name:

PE anti-human CD15

Catalog Number:

301905

Clone Number:

HI98

Info:Supplier Page View Product Specs

Browse Similar Products: CD15 Antibodies

We used this antibody in a panel for classification of MDSC isolated from whole blood by Ficoll density gradient centrifugation. Stains Granulocytes in whole blood and MDSC. Not sure about IF, however, never tried that.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC

Primary Incubation

1:100

Blocking Agent

BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Classification of MDSC in PBMC: Combinaed with other antibodies rather easy as this antibody provides a distinct signal when cells are positive compared to CD15 negative cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Quick, easy, cost-efficient

The Bad

None

The Bottom Line

Easy to use, quick, efficient and affordable.

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