Zymo Research
Anti-5-Methylcytosine Monoclonal Antibody
A3001
Clone 7D21
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Browse Similar Products: 5-Methylcytosine Antibodies
We were interested in studying the role of DNA methylation in CD4 T-cells. A variety of antibodies where used to measure different DNA methylation markers(5mC, 5hmC, 5fC, 5caC, 6ma) during their differentiation.
Immuno Dot Blot
gDNA
1:3000, 1h on Ice
Casein buffer
1:5000
N/A
ChemiDoc MP Syste
Synthetic oligo-probes, containing only 1 type of methylated cytosine (5mc/5hmc/5fc/5cac) or methylated adenosine (6ma) where stained with the a-5mC antibody. The results show that the antibody is specific to 5mC only.
DOI: 10.1126/sciadv.aay3335
Easy to use, works well.
A bit expensive.
The antibody is very specific to each target and provides a strong staining signal when used in an in-direct immunodotblot assay.