Cell Signaling Technology
Phospho-EGF Receptor (Tyr1068) Antibody
#2234
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Good antibody that recognises phosphorylation of the EGFR at Y1068, following treatment of MCF10a cells with EGF for 15 minutes.
Western Blot
MCF10a cells`
1:1000, overnight at 4 degrees
10% milk in TBST
1:2000 in 10% milk in TBST
ECL detection
Great antibody - found antibodies that recognise receptors can be quite messy sometimes, but after a 2.5 hour semi-wet transfer, this antibody detects the protein well with little background noise.
One of the better EGFR antibodies out there.
Quite expensive.
Would buy again.