Invitrogen
Calcein AM
C3100MP
Info:Supplier Page
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases. Is working very well for flowcytometry to assess cell viability and also for immunofluorescence staining to show viable cells at the microscope
Cell viability
N/A
Prepare aliquots from the stock stable for 3-6 month at -20.
Calcein, AM has been used for studies of enzymatic activity, cell membrane integrity, and long-term cell tracking due to its low cellular toxicity. It is important to note that for certain cell lines and experimental conditions, relatively high concentrations of Calcein, AM can be toxic to cellsi usually use 2 micromolar solution (1ul in 500ul of PBS) for both flow and immunofluorescence staining starting from a stock of 1mM.
Very sensitive and specific signal for flow cytometry and immunofluorescence.
None, overall the only issues depend on the quality of sample.
Overall very good for flow and immunofluorescence to detect viable cells.