Miltenyi Biotec
CD159a (NKG2A) Antibody, anti-human, PE, REAfinity™
130-113-566
REA110
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I wanted to stain the expression of NKG2A on the surface of CD56bright NK cells in some different contexts, so I therefore stained human PBMCs in serial dilutions from 1:25, 1:50, 1:100, 1:200, 1:400 and 1:800 respectively from left to right as seen in the image attached.
Flow Cytometry
Human PBMC
30 minutes at room temperature
PBS/BSA
None
yg-586/15
This antibody works super well and is even good at high titration - I will use at 1:800 in the future. Very distinct populations.
N/A
Extremely bright.
Nothing!
I will use this in my phenotyping panels going forward.