Anti-C1q Antibody Detects Activation of Complement in Mouse Brain Tissues

September 30, 2022

Albert Einstein College of Medicine

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-C1q

Catalog Number:

ab182451

Clone Number:

[4.8]

Info:Supplier Page View Product Specs

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We employ Abcam's anti-C1Q monoclonal [4.8] to detect activated complement in the brain tissues of our murine models of aging and neurodegeneration. The antibody requires optimization prior to use but once the workflow is finalized provides reliable, repeatable results.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain sections

Primary Incubation

1:200 O/N @ 4 degrees C

Blocking Agent

BSA/Serum

Secondary Incubation

Alexa conjugated goat anti-rabbit; 1:500 for 1 hour @ RT

Tertiary Incubation

N/A

Detection

Fluorescent or confocal microscopy

Results Summary

The antibody provides clear staining once optimized, but it requires a higher concentration of serum and BSA than standard protocols. This is important to ensure reliable and repeatable results.

DOI or PMID #

N/A

Additional Notes

Very few publications include well described protocols using this reagent (complete details for use are lacking in the literature).

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Summary

The Good

Provides reliable and repeatable results once optimized.

The Bad

Takes time to optimize, especially given lack of details regarding correct use in the literature.

The Bottom Line

If you have time to fully optimize and trial this reagent, we recommend it for use in detecting activated complement in murine (brain) tissues.

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