Good DAPI Reagent

Results Summary

Mouse heart was fixed in 4% paraformaldehyde. The fixed heart tissues were dehydrated and embedded in paraffin. For immunofluorescence staining, heart sections were incubated with anti-CD45 and anti-F4/80 antibody (1:100, Cell Signaling Technology, USA) at 4◦C overnight. After 3 times wash with PBS, fluorescein-isothiocyanate-conjugated secondary antibodies (1:1,000, Cell Signaling Technology, USA) were incubated at room temperature in the dark for 1 h. After DAPI (Sigma-Aldrich, USA) staining, fluorescence images were captured under the fluorescence microscope (Leica, Wetzlar, Germany). Representative immunofluorescence images showed the CD45+ inflammatory cells and CD45+F4/80+ macrophages in the heart.