FLAG Antibody Works Well for WB

September 19, 2022

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

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Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

DYKDDDDK Tag (D6W5B) Rabbit mAb

Catalog Number:

14793S

Clone Number:

D6W5B

Info:Supplier Page View Product Specs

Browse Similar Products: FLAG Antibodies

HEK293 cell were transfected with vector including flag-cas9 sequence (PX458). After 48 hrs, cell pellets were collected for detecting flag-cas9 expression. The results clearly showed the antibody specifically detected flag-cas9 expression in PX458-transfected cell but not control WT cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HEK293 cell

Primary Incubation

1:2000 overnight at 4 degree

Blocking Agent

5% milk in TBS

Secondary Incubation

1 hour at room temperature

Tertiary Incubation

1:5000

Detection

ECL

Results Summary

HEK293 cell were transfected with vector including flag-cas9 sequence (PX458). Western blot using this antibody showed the antibody specifically detected flag-cas9 expression in PX458-transfected cell

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Antibody works well in WB.

The Bad

N/A

The Bottom Line

This antibody works great, highly recommended.

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