Module Used for Jess Assay

University of Texas Medical Branch Galveston

Overall

Quality of Results

Ease-of-Optimization

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Company:

ProteinSimple

Product Name:

12-230 kDa Separation Module

Catalog Number:

SM-W004-1

Currently working with in vitro cell culture and exosomes, doing some experiments that results in low amount of protein in cell lysates. Jess assay is an option for this type of situation.

Experimental Design and Results Summary

Application

Protein analysis

Starting Material

Cell Lysate

Tips

25 capillary cartridge (1 MW 24 samples)

Results Summary

After optimization, I was able to run 24 samples at once, with 3 antibodies in the same capillary, saving the amount of cartridge used.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Low amount of protein required; running 3 antibodies in the same run.

The Bad

Some antibodies are difficult to optimize.

The Bottom Line

In summary, this is a good option for experiments that did not give you enough amount of protein to run a regular western blot. Also, since the run is all automated, you just need to take a time to prepare all the samples and the antibodies, and the run will give you the results in 3 hours.

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