Very Specific Antibody

Yale institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

RABBIT POLYCLONAL TO DERL1/DERLIN-1

Catalog Number:

ab176732

Clone Number:

N/A

Derlin-1 is a protein located in the ER membrane. The levels of this protein was checked in cancer cells lines to determine if there is any basal level changes in the expression of DERLIN-1 in different cell lines through western blot. the antibody gives very specific band with no background.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cancer cell lines

Primary Incubation

1:2000 in 5% milk TBST overnight at 4 degree.

Blocking Agent

5% milk in TBST

Secondary Incubation

1:2000 IN 1% MILK-TBST

Tertiary Incubation

N/A

Detection

X RAY FLIMS

Results Summary

The western blot shows that DERLIN-1 expression varies at basal levels in different cancer cell lines.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very specific.

The Bad

The product is not bad.

The Bottom Line

The antibody detects very specific band with no background. Recommended.

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