Antibody Stains hCD163 Very Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cyanine7 anti-human CD163 Antibody

Catalog Number:

326514

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: CD163 Antibodies

We wanted to phenotype human macrophages in an in vitro culture, so we used hCD163 as a differentiation marker to compare to monocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

In vitro differentiated human macrophages

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT cytometer

Results Summary

The antibody clearly stains hCD163, consistent with an M2 macrophage phenotype.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great.

The Bad

None to mention.

The Bottom Line

Human M2 macrophages can be well identified using this anti-hCD163 antibody.

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