Cell Signaling
Akt Antibody
9272
N/A
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I evaluated the total amount of AKT on A549 (epithelial lung cells) transfected for 24 hours with eIF4E siRNA. I got great results, and all my cells showed the same amount of AKT. I diluted the antibody with 5% BSA in TBST 1:1000 (v/v).
Western Blot
Epithelial cells
1:1000, 4 °C, overnight
BSA 5% TBST
1:10000
Chemiluminescence
I evaluated the total amount of AKT on A549 (epithelial lung cells) transfected for 24 hours with eIF4E siRNA. I got great results all my cells showed the same amount of AKT. I diluted the antibody with 5% BSA in TBST 1:1000 (v/v).
Band is clear.
Antibody has great signal, highly recommended.