Cell Signaling
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb
9145
N/A
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I seeded epithelial cells with 10% FBS. The day after, I replaced the medium (starvation medium, 0.5%) and treated it with eIF4E siRNA for 24 hours. Though a bit expensive, band was clear. Would purchase again.
Western Blot
Epithelial cells
1:1000, 4 °C, overnight
BSA 5% TBST
1:10000
Chemiluminescence
I seeded epithelial cells with 10% FBS. The day after I replaced the medium (starvation medium, 0.5%) and I treated with eIF4E siRNA for 24 hours.
Band is clear.
Expensive but great, would buy again.