Cell Signaling Technology
GAPDH (D16H11) XP Rabbit mAb
5174S
N/A
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We have been using this antibody to detect GAPDH levels in cell lysates. The antibody is very specific and works well at the suggested concentration.
Western Blot
Mammalian cell line whole cell lysate
1:1000, overnight incubation at 4 degree Celsius
5% BSA in TBST
Goat anti-rabbit IgG HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT
ECL
Antibody reacted well. Ver few non-specific bands. Overall it's a very good antibody to detect the expression of GAPDH. I recommend it.
Quality of the results and signal.
Minor background signals.
Works well for western blotting to detect GAPDH expression at the suggested dilution.