Good but Slightly Faint Antibody for WB

May 11, 2022

Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

IL-6 antibody

Catalog Number:

12153S

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Used this antibody on human astrocytes at 1:1000 dilution. Resulted in a number of fairly weak bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human astrocytes

Primary Incubation

1:1000 overnight BSA

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Tried to demonstrate astrocyte activation via amyloid-beta using IL-6 levels. Bands are in the correct place but I was unable to differentiate between treatments.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works okay.

The Bad

Bands could be stronger.

The Bottom Line

Not bad, but not great.

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