Abcam
Anti-UCP1 antibody
ab10983
Polyclonal
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I used this antibody for detecting UCP1 in the iBAT of UCP1 KO and WT mice.
Western Blot
iBAT (interscapular brown adipose tissue)
1:2000 for 1 hour at room temperature
Bio-Rad EveryBlot Blocking buffer
1:10,000, 1 hour at room temperature (StarBright 700)
No tertiary incubation
Bio-Rad ChemiDoc MP Imaging System
This Ab specifically detected UCP1 in the 5 micrograms of iBAT lysate.
https://doi.org/10.1038/s41467-020-18865-z
N/A
Good for Western blot.
Nothing bad.
This Ab was only tested by WB and immunofluorescence staining.