Thermo Fisher
Insulin
14-9769-82
ICBTACLS
Info:Supplier Page View Product Specs
Browse Similar Products: Insulin Antibodies
We are interested to study tissue-resident macrophages in various, including the pancreas. For this, we wanted to identify the endocrine and exocrine pancreas regions.
Immunofluorescence
PLP fixed 14 μm cryosections of mouse pancreas from a 10 week old C57BL/6J mouse
1:100 overnight at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% goat serum, 2% rat serum
1:500 goat anti-mouse alexa fluor 647 1h at room temperature
N/A
Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.
Great staining of pancreatic islets, with little background. Yet, since this is a mouse antibody, it could give high background in some cases/conditions.
Great staining of pancreatic islets.
Expensive. Mouse primary antibody.
Highly recommended.