BD Pharmingen
FITC Annexin V
556420
N/A
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FITC Annexin V binds to phosphatidylserine and enables the identification of procoagulant platelets, which express phosphatidylserine and participate in coagulation. Annexin V staining can be challenging and requires the addition of calcium to staining buffers.
Flow Cytometry
Human whole blood collected in sodium citrate
30 min at room temperature
Staining buffer contains 0.5% BSA
Fluorescence
Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing (1) 2.5 mM calcium to facilitate FITC Annexin V binding, (2) 2.5 mM Gly-Pro-Arg-Pro (GPRP) to inhibit clot formation, (3) 1 µg/mL FITC Annexin V, and (4) 10 µg/mL CRP-XL and 10 µM TRAP (platelet agonists) to induce platelet activation and phosphatidylserine exposure. Lyse/fix buffer was added, and samples were acquired by flow cytometry. FITC Annexin V allowed clear resolution of procoagulant platelets.
Allows clear resolution of procoagulant platelets.
FITC Annexin V allows clear resolution of procoagulant platelets.