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Thy1.2 BV570
105329
30-H12
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We use single cell analysis methods to study the mechanisms responsible for establishing and maintaining immune tolerance.
Flow Cytometry
Mouse thymus, spleen, lymph nodes
Block: 1:50 in 100uL at 37C for 30 min
Fc Block (aCD16/32)
L/D: Zombie NIR 1:4000 in 100 uL at RT for 20 min
Surface stain: 1:400 in 100uL at 4C for 30 min
Spectral flow cytometry (Cytek Aurora)
Great antibody to get a very clean separation of all T cells from other immune cell populations in a single gate. Can be used to remove T cells to analyze other cell populations or to pregate before subsetting T cells into different subpopulations using other antibodies.
Cheap, great staining.
None
A great antibody to identify T cells in spectral flow cytometry.