Cell Signaling Technology
anti-Jak2
3230
D2E12
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The lab focus is on the study of pediatric leukemia in the context of JAK-STAT signaling. We are interested in developing new therapies and treatments for this disease. We are also interested in drug resistant leukemia.
Western Blot
Human MUTZ-5 B-ALL cells
Incubate primary antibody in 5% BSA in TBST overnight with an oribital shaker at 4*C
5% BSA in TBST
Incubate secondary antibody in 5% BSA in TBST for one hour with an orbital shaker at room temperature
N/A
Amersham ECL Prime Western Blotting Detection Reagent (Cytiva RPN2236)
MUTZ-5 cells were treated with several JAK2 inhibitors overnight. Total JAK2 was used as a loading control.
Great signal strength
Not really any downsides
This is a good antibody to use when you are interrogating JAK-STAT signaling in your cell of choice. It gives a nice clean signal quickly.