High Variability Between Reads

CNC
Biotech
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Fluorescence Biotin Quantitation Kit

Catalog Number:

46610

Easy to perform with quick results using a low volume of sample. However, there is high variability between reads, including calibration curves with biocytin control.

Experimental Design and Results Summary

Application

Biotin quantification

Starting Material

Tips

Incubate for 5 minutes, RT

Results Summary

Biotin quantification was within range after dilution of samples 1:800 in PBS

DOI or PMID #

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Summary

The Good

Fast results

The Bad

High variability

The Bottom Line

Need optimization to quantify sample within the range of the calibration curve

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