Goat Anti-Iba1 Antibody for Immunofluorescence

January 12, 2022

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Wako

Product Name:

Goat Anti-Iba1 Antibody for Immunofluorescence

Catalog Number:

011-27991

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-Iba1 Antibodies

Compared to other Iba1 antibodies, I would not recommend this antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse spinal cord

Primary Incubation

1:200, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope.

Results Summary

Using different Iba1 antibodies simultanesouly, I observed that this antibody performs worse than the others. From the same company, the rabbit Iba1 is highly recommended.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

None.

The Bad

Quality.

The Bottom Line

I would recommend to use other Iba antibody.

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