Consistent CD19 for all B Cells

Pathology
UMass
Lab Technician
December 03, 2021

Pathology
UMass
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Beckman Coulter

Product Name:

CD19-ECD, J3-119, 1 mL, ASR

Catalog Number:

IM2708U

Clone Number:

J3-119

Info: Supplier Page View Product Specs

Browse Similar Products: CD19 Antibodies

CD19 is the typical marker for B cells. It is preferable to CD20 that is only expressed on mature B cells. Our lab investigates B cells at all stages of maturation. We therefore need a good antibody to ensure all B cells are identified.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood (100uL)

Primary Incubation

15-30 minutes at room temp

Blocking Agent

1% FBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Navios flow cytometer

Results Summary

CD19 ECD from Coulter gives us a good signal and does not require too much optimization. However, ECD (PE-Texas Red) spills heavily into neighboring channels which means a fair amount of compensation is needed.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

A good signal, yielding acceptable resolution of positive versus negative cells

The Bad

As an ECD conjugate, spillover/spread becomes an issue

The Bottom Line

This antibody is a good anti-CD19 reagent, that is also ASR which is preferable in clinical trials. Compensation can be an issue, and must be done properly.

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