Great Streptavidin Stain

Surgery
UCSF
Graduate Student
November 19, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Alexa Fluor® 594 Streptavidin

Catalog Number:

405240

Clone Number:

N/A

Streptavidin conjugates are used to detect biotin stained markers. We use it to detect CCR7 biotin stained samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:100 dilution for 30 minutes at 37 degrees C

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

30 minutes with streptavidin at 4 degrees C

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

Allowed us to see that many antigen presenting cells in mouse lymph node are indeed CCR7+.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Stains cleanly and reliably

The Bad

Requires secondary incubation

The Bottom Line

Great choice for a streptavidin conjugate, will use again.

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