MyBioSource.com
DGAT2 Antibody
MBS9407865
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Mammalian smooth cells in the log phase of growth were synchronized, and for a different period, these cells were treated with a specific growth factor. The cell extract prepared from these treatments was resolved in 12 % SDS-PAGE and western transferred on the nitrocellulose membrane. Next, the blot was detected to measure the effect of the growth factor treatment on DGAT2 using its specific antibody obtained from MyBioSource.com. The selection of antibody was based on western blot picture information provided in the antibody flyer.
Western Blot
Mammalian whole-cell protein lysate
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-rabbit IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray film
MyBioSource.com antibody has detected DGAT2 with high specificity with mammalian protein samples. No nonspecific protein band was detected.
A highly specific band of DGAT2 were observed
Excellent antibody for Immunodetection of DGAT2 in mammalian cells