MyBioSource.com
Anti-Mannose 6 Phosphate Receptor/Igf2r Antibody
MBS1751289
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The mouse which was subjected to treatment was euthanized, collected tissue and protein collected from tissues were examined to test the effect of the treatment on mice on Igf2r protein levels. In brief, extracted proteins from these tissues were resolved in 8% SDS-PAGE and immunoblotted for Igf2r using MyBioSource.com Ab. The selection of Igf2r Ab was based on the antibody flyer.
Western Blot
Mouse tissue protein extract
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degrees Celsius
5% skimmed milk made in 1x TBST
Goat anti-Rabbit IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray film
The antibody has detected a single and specific band of Igf2r protein at nearly 270 kDa.
It gives a highly specific band. Not a single band of nonspecific epitope was observed
Excellent antibody