Invitrogen
Mouse monoclonal anti V5 antibody
R960-25
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Browse Similar Products: Anti-V5 Tag Antibodies
1:1000 dilution as primary antibody always always gives strong and very specific signal in WB. Great antibody!
Western Blot
293t cell lyastes
1:1000 overnight in cold room
Odyssey blocking buffer
Goat at mouse:680
1:10000 room temperature for 1hr
LiCOR
The antibody shows one band from cell lysates and the intensity of band gets strong as more DNA is transfected into cells.
Everything is good
Nothing bad
Great antibody.