V5 Tag Antibody R960-25 from Thermo Fisher Scientific

Product Specs
ItemV5 Tag Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR960-25
Quantity50 µL
ApplicationsELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate/TagUnconjugated
FormatPBS with 0.01% sodium azide
ReactivityTag
TargetV5 Tag
IsotypeIgG2a
HostMouse
Antibody TypePrimary
Regulatory StatusRUO - research use only
ImmunogenV5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-
Brand/TrademarkNovex®
ClonalityMonoclonal
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles
CloneSV5-Pk1

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) The HSP40 family chaperone isoform DNAJB6b prevents neuronal cells from tau aggregationBMC BiolDecember 18, 2023Ya-Lan Chang, Chan-Chih Yang, Yun-Yu Huang, Yi-An Chen, Chia-Wei Yang, Chia-Yu Liao, Hsun Li, Ching-Shyi Wu, Chin-Hsien Lin, Shu-Chun Tenga humidity incubator. After blocking, cells were incubated with primary antibodies (anti-V5, 1:250, R960-25, Thermo Fisher Scientific, and anti-tau, 1:250, GTX112981, Genetex) overnight at 4℃. On the next day,

(2) Galectin-1 Modulates the Fusogenic Activity of Placental Endogenous Retroviral EnvelopesVirusesDecember 16, 2023Caroline Toudic, Maike Maurer, Guillaume St-Pierre, Yong Xiao, Norbert Bannert, Julie Lafond, Éric Rassart, Sachiko Sato, Benoit BarbeauPBS) was added for 45 min at RT, and, after addition of mouse monoclonal anti-V5 antibodies (1/500, R960-25, Thermo Fisher Scientific), cells were incubated overnight at 4 °C with shaking. Following three washes

(3) Wild-type C-Raf gene dosage and dimerization drive prostate cancer metastasisiScienceDecember 15, 2023Lisa Ta, Brandon L. Tsai, Weixian Deng, Jihui Sha, Grigor Varuzhanyan, Wendy Tran, James A. Wohlschlegel, Janai R. Carr-Ascher, Owen N. Witteantibodies. The following antibodies were used to detect the corresponding proteins V5 (Invitrogen R960-25; 1:2,500); BRAF (Cell Signaling 55C6; 1:1,000); ARAF (Cell Signaling 4432S; 1:1000); C-Raf (Sigma HPA002640;

(4) Xport-A functions as a chaperone by stabilizing the first five transmembrane domains of rhodopsin-1iScienceDecember 15, 2023Catarina J. Gaspar, Tiago Gomes, Joana C. Martins, Manuel N. Melo, Colin Adrain, Tiago N. Cordeiro, Pedro M. DomingosPVDF membranes (Amersham Hybond) and probed with the following antibodies: mouse anti-V5 (1:1,000) (R960-25, Invitrogen), mouse anti-TRP (1:300) (Mab83F6, DSHB), mouse anti-Rh1 (1:200) (4C5, DSHB), rat anti-Xport-A

(5) Control of CCR5 Cell-Surface Targeting by the PRAF2 GatekeeperInt J Mol SciDecember 13, 2023Elisa Da Silva, Mark G. H. Scott, Hervé Enslen, Stefano Marullorabbit polyclonal antibody (ab53113; Abcam, Amsterdam, Netherlands), anti-V5 mouse monoclonal antibody (R960-25; Thermo Fischer, Villebon-sur-Yvette, France), anti-Rluc mouse monoclonal antibody (MAB 4410-I, Roche,

(6) RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophagesbioRxivDecember 7, 2023Teodorus Theo Susanto, Victoria Hung, Andrew G. Levine, Craig H. Kerr, Yongjin Yoo, Yuxiang Chen, Juan A. Oses-Prieto, Lisa Fromm, Kotaro Fujii, Marius Wernig, et al.(Cell Signaling Technology, 3700S); Rps6 (Cell Signaling Technology, 2217); V5 Tag (Thermo Fisher, R960–25); GAPDH (D16H11) (Cell Signaling Technology, CS5174S); Llph (Invitrogen, PA5–66207); Rps20 (Proteintech,

(7) Luminal breast cancer identity is determined by loss of glucocorticoid receptor activityEMBO Molecular MedicineDecember 7, 2023Stefan Prekovic, Theofilos Chalkiadakis, Merel Roest, Daniel Roden, Catrin Lutz, Karianne Schuurman, Mark Opdam, Liesbeth Hoekman, Nina Abbott, Tanja Tesselaar, et al.mM KH2PO4, 2.6 mM KCl, and 0.1% Tween‐20) for 1 h and incubated with primary antibodies against V5 (R960‐25, ThermoFisher) diluted in 3% BSA/PBS‐T for 2 h. After three washing steps in PBS‐T, membranes were incubated

(8) Carboxypeptidase A4 negatively regulates HGS-ETR1/2-induced pyroptosis by forming a positive feedback loop with the AKT signalling pathwayCell Death DisDecember 4, 2023Luoling Wang, Rilin Deng, Shuishun Chen, Renyun Tian, Mengmeng Guo, Zihao Chen, Yingdan Zhang, Huiyi Li, Qian Liu, Songqing Tang, et al.purchased from Merck Millipore. Flag (F3165) and β-actin (A5441) were purchased from Sigma-Aldrich. V5 (R960-25) was purchased from Thermo Fisher Scientific. Normal mouse IgG (sc-45123) was purchased from SANTA CRUZ.

(9) Nanoscaled RIM clustering at presynaptic active zones revealed by endogenous taggingLife Science AllianceDecember 1, 2023Achmed Mrestani, Sven Dannhäuser, Martin Pauli, Philip Kollmannsberger, Martha Hübsch, Lydia Morris, Tobias Langenhan, Manfred Heckmann, Mila M Paulmonoclonal α-HA (1:500; C29F4 catalog #3724; Cell signaling technology), and mouse monoclonal α-V5 (1:100; R960-25; Invitrogen). Secondary antibodies were used in the following concentrations: goat α-rabbit conjugated

(10) Mass isolation of staged Drosophila pupal intestines for analysis of protein ubiquitylationSTAR ProtocolsDecember 1, 2023Ruoxi Wang, Panagiotis D. Velentzas, Eric H. Baehreckeor RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, "V5 (1:1,000)",Invitrogen,"Cat#R960-25, RRID: AB_2556564" "Ubiquitin (P4D1) (1:1,000)",Cell Signaling Technology,"Cat#3936, RRID: AB_331292" "Actin

(11) Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysisJournal of virologyNovember 30, 2023Pahmeier F, Lavacca T-M, Goellner S, Neufeldt CJ, Prasad V, Cerikan B, Rajasekharan S, Mizzon G, Haselmann U, Funaya C, et al.anti-HA,,1:500,Invitrogen,Cat.#PA1-985 Mouse IgG2a anti-V5,"1:1,000",1:500,ThermoFisher Scientific,Cat.#R960-25 Rabbit anti-PDI,,1:100,Sigma Aldrich,Cat.#P7372 Mouse anti-RTN3,,1:100,Santa Cruz Biotechnology,Cat.#sc-374599 Rabbit

(12) Electrical synapse structure requires distinct isoforms of a postsynaptic scaffoldPLOS GeneticsNovember 27, 2023Jennifer Carlisle Michel, Margaret M. B. Grivette, Amber T. Harshfield, Lisa Huynh, Ava P. Komons, Bradley Loomis, Kaitlan McKinnis, Brennen T. Miller, Ethan Q. Nguyen, Tiffany W. Huang, et al.clone 5C10A, 1:350), mouse IgG1 anti-ZO1 (Invitrogen, 33–9100, 1:350), mouse IgG2a anti-V5 (Invitrogen, R960-25, 1:500), rabbit anti-human Cx36 (Invitrogen, 36–4600, 1:500) and chicken anti-GFP (Abcam, ab13970, 1:350–1:500).

(13) Hypoxia-induced transcriptional stress is mediated by ROS-induced R-loopsNucleic Acids ResearchNovember 27, 2023Tiffany S Ma, Katja R Worth, Conor Maher, Natalie Ng, Chiara Beghè, Natalia Gromak, Anna M Rose, Ester M HammondG9a (3306), H3 (14269), H3K9me2 (4658), KAP-S824 (4127), p53-S15 (9284), PERK (3192); Invitrogen: V5 (R960-25); Millipore: γ-H2AX (05–636), H2AX (07–627), H3K9me3 (07–523); Santa Cruz: β-actin (AC-15), ATR (sc-515173),

(14) Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56Nature CommunicationsNovember 16, 2023Johnny Lisboa, Cassilda Pereira, Rute D. Pinto, Inês S. Rodrigues, Liliana M. G. Pereira, Bruno Pinheiro, Pedro Oliveira, Pedro José Barbosa Pereira, Jorge E. Azevedo, Dominique Durand, et al.rabbit polyclonal antibody (sc-372, dilution 1:3000) was from Santa Cruz Biotechnology and the anti-V5 (R960-25, dilution 1:5000) mouse monoclonal antibody was purchased from Invitrogen. Goat anti-IgG rabbit alkaline

(15) Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophaseDevelopmentNovember 15, 2023Catherine C. Baker, Lorenzo Gallicchio, Neuza R. Matias, Douglas F. Porter, Lucineh Parsanian, Emily Taing, Cheuk Tam, Margaret T. Fullerused for anti-GFP (mouse, Millipore Sigma #11814460001) and anti-V5 (mouse, Thermo-Fisher Scientific, #R960-25) (2 µl undiluted antibody was used per 30 µl of suspended beads per immunoprecipitation). The 6 Tube

(16) Genetically proxied HTRA1 protease activity and circulating levels independently predict risk of ischemic stroke and coronary artery diseaseResearch SquareNovember 8, 2023Martin Dichgans, Rainer Malik, Nathalie Beaufort, Koki Tanaka, Marios Georgakis, Yunye He, Masaru Koido, Chikashi Terao, Christopher Anderson, Yoichiro Kamatanioverexpressing HTRA1 (20 μl) for 24 h at 37°C and samples were analyzed by anti-V5 immunoblot (Invitrogen, #R960–25, 1:10,000). Where appropriate, wt or mutant HTRA1-containing secretome was diluted in secretome from

(17) HCMV UL8 interaction with β-catenin and DVL2 regulates viral reactivation in CD34 + hematopoietic progenitor cellsJournal of VirologyOctober 31, 2023Aaron Dirck, Nicole L. Diggins, Lindsey B. Crawford, Wilma D. Perez, Christopher J. Parkins, Hillary H. Struthers, Rebekah Turner, Andrew H. Pham, Jennifer Mitchell, Courtney R. Papen, et al.Promega, CS2006A01), FLAG M2 (Sigma, F3165), anti-FLAG (Cell Signaling, D6W5B), V5 Tag (Thermo Fisher, R960-25), V5-tag (D3H8Q, Cell Signaling, #14793), GAPDH Ab (6C5) (Abcam, ab8245), NanoGlo HiBiT Blotting System

(18) Biased eviction of variant histone H3 nucleosomes triggers biofilm growth in Candida albicansmBioOctober 31, 2023Priya Brahma, Rashi Aggarwal, Kaustuv Sanyalantibody) and mock (− antibody). To the IP fraction, 3 µL of anti-V5 antibody (Invitrogen, catalog number R960-25) was added, and the tubes were incubated at 4°C overnight on rotation. A 30-µL of Protein-A beads (Sigma,

(19) A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical in Sinorhizobium meliloti for symbiosis with legume hostsPLOS GeneticsOctober 23, 2023Julian A. Bustamante, Josue S. Ceron, Ivan Thomas Gao, Hector A. Ramirez, Milo V. Aviles, Demsin Bet Adam, Jason R. Brice, Rodrigo A. Cuellar, Eva Dockery, Miguel Karlo Jabagat, et al.membrane for detection by chemiluminescence (SuperSignal West Pico). Monoclonal anti-V5 (Invitrogen R960-25; AB_2556564) was used at 1:2500 dilution, anti-HA [clone 2–2.2.14] (Thermo 26183; AB_10978021) used

(20) Notch Signaling Regulates Mouse Perivascular Adipose Tissue Function via Mitochondrial PathwaysGenesOctober 20, 2023Yang C, Yang X, Harrington A, Potts C, Kaija A, Ryzhova L, Liaw L.mg/mL RBPJ-k,Cell Signaling,5313,0.025 mg/mL UCP1,Cell Signaling,14670,0.4 mg/mL V5 tag,ThermoFisher,R960-25,0.204 μg/mL Antibodies. Vendors include Cell Signaling (Danvers, MA, USA), BD Biosciences (Franklin

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(1)

9/24/2021Good Mouse Monoclonal Anti-V5 Antibody Wei Wei1:1000 dilution as primary antibody always always gives strong and very specific signal in WB. Great antibody!
(1)

7/16/2019Fantastic Antibody to Detect V5-Tagged Recombinant Proteins (Thermo) Aleksandra WroblewskaExcellent antibody, highly recommended!
(1)

2/27/2017Test Of V5-CRBN Plasmid For Transfection Liyong Z.Different cyclic-di-peptides were used to test if it could affect the expression of cereblon in HCT116 cells. HCT116 were transfected with the V5-CRBN plasmids using lipofectamine 2000. The expression... Read Review

V5 Tag Antibody

V5 Tag Antibody

V5 Tag Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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V5 Tag Antibody from Thermo Fisher Scientific

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