Biolegend
APC anti-human NGFR (CD271)
345108
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The antibody works really well for the IL-17A-hNGFR reporter mice. The surface staining for hNGFR is really strong and no intracellular staining is required to measure IL-17A production.
Flow Cytometry
In vitro mouse Th17 differentiation
1:100 in FACS Buffer at RT for 30 min
N/A
Great resolution from negative vs. positive populations.
Consistent results across different batches
Only works for flow.
Expensive but great. In order to keep data consistent, our lab purchases this antibody frequently.