SNAI Antibody for Electro Mobility Shift Assay (EMSA) and Supershift EMSA

July 24, 2021

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz

Product Name:

SNAI1

Catalog Number:

sc-271977

Clone Number:

N/A

Zinc finger protein SNAI1 is a protein that in humans is encoded by the SNAI1 gene. Snail is a family of transcription factors that promote the repression of the adhesion molecule E-cadherin to regulate epithelial to mesenchymal transition (EMT) during embryonic development. Here we checked the effect of chemoattractant on SNAI1 EMSA and super shift EMSA.

Experimental Design and Results Summary

Applications

EMSA

Sample

Mouse Lysate

Primary Incubation

1:200 dilution and incubate with labelled probe

Blocking Agent

Blocking Buffer provided along with the EMSA kit

Secondary Incubation

None

Tertiary Incubation

None

Detection

ECL

Results Summary

We found supershift of SNAI1 in chemoattractant treated sample by EMSA.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This is a high concentration antibody and works great for EMSA

The Bad

None

The Bottom Line

Great antibody for EMSA and supershift EMSA. Highly recommended.

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