BD Biosciences
PE Rat Anti-Mouse CD274
558091
MIH5
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Browse Similar Products: Anti-PD-L1 Antibodies
As a cancer immunology lab, we focus on T cells in the context of tumor immunity. We use this antibody for multicolor flow cytometry to identify PDL1 cells in different immune cells but also tumor as it plays an important role in immune evasion.
Flow Cytometry
Murine tumor
1:500 in FACS Buffer (2% FBS/PBS) 30 minutes at 4deg
FC Block before staining in FACS 10 minutes at 4deg
N/A
Cytek Aurora (4 Laser)
We can detect bright expression of PDL1 not on T cells but other cells in the tumor.
Titrate before actual use
Bright staining and cheap for amount of antibody
None
Highly recommend this antibody to detect PDL1 expression on cells.