Good Antibody to Detect Mouse CD4

July 24, 2021

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV563 Rat Anti-Mouse CD4

Catalog Number:

612923

Clone Number:

GK1.5

As a cancer immunology lab, we focus on T cells in the context of tumor immunity. We use this antibody for multicolor flow cytometry to identify CD4 T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine splenocytes

Primary Incubation

1:500 in FACS Buffer (2% FBS/PBS)

Blocking Agent

FC Block before staining in FACS

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Cytek Aurora (4 Laser with UV)

Results Summary

We can readily detect a bright population of CD4+ T cells in the spleen.

DOI or PMID #

N/A

Additional Notes

Titrate

Image Gallery

Summary

The Good

Ready to purchase UV antibody from BD

The Bad

Expensive

The Bottom Line

Highly recommend this antibody for flow if using the UV laser.

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