Wortmannin Works Well to Inhibit PI3K

April 19, 2021

Johns Hopkins University School of Medicine
Department of Cell Biology
Graduate Student

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Company:

Sigma-Aldrich

Product Name:

Wortmannin, (PI3 Kinase inhibitor)

Catalog Number:

12-338

Info: Supplier Page 

We used this Wortmannin to inhibit PI3K and other it's role in other phospholipids or signaling molecules.

Experimental Design and Results Summary

Application

Cell assay

Starting Material

N/A

Tips

Perform the experiment according to manufacturer's instructions.

Results Summary

PI3K and PIP3 are inhibited after Wortmannin treatment within 5 minutes. As shown in the image, PIP3 (red) biosensor fall off the membrane after PI3K inhibition, but PI(3,4)P2 biosensor remains on the membrane

DOI or PMID #

N/A

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Summary

The Good

Reliable, high quality, user friendly

The Bad

None

The Bottom Line

I highly recommend this reagent to inhibit PI3K

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