HCAM Antibody Useful for Evaluating CD44 Expression by IHC

January 09, 2021

BlueskyReddit
Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz

Product Name:

HCAM Antibody

Catalog Number:

sc-7297

Clone Number:

N/A

HCAM is also known as CD44. Human and mouse breast cancer specimens were probed for HCAM by using immunohistochemistry to evaluate co-staining of cancer stem cell markers.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human and mouse breast cancer tissue

Primary Incubation

1:250, overnight at 4 degrees

Blocking Agent

5% Donkey Serum

Secondary Incubation

Donkey Anti-mouse Alexa 488, 1 hour 1:100

Tertiary Incubation

N/A

Detection

Microscopy (Immunofluorescence)

Results Summary

HCAM (CD44) was localized to the cellular membrane as expected and did not show total staining of all cells as was observed with other CD44 antibodies.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, good staining

The Bad

May need some optimization

The Bottom Line

The HCAM antibody appears to to be reliable for IHC/flow cytometry- based staining. Others antibodies exhibit complete staining of all cells which is not reliable. However, this antibody appears to be more specific.

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