Immunostaining for Axonal Fibers

October 01, 2020

BlueskyReddit
Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

DSHB (Developmental Studies Hybridoma Bank)

Product Name:

Neurofilament-M

Catalog Number:

2H3

Clone Number:

N/A

NFM-2H3 antibody was used to stain axonal fibres in the mouse embryonic brain cortex at E16

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

frozen embryonic mouse brain tissue (E16) and thick slices of 500 microns

Primary Incubation

1:150, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

NFM antibody was used to label axonal fibres in the embryonic brain. specific signal was obtained-

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific staining, good for thinner sections

The Bad

None, sometimes may cross-react with blood vessels-care must be taken to avoid this

The Bottom Line

Specific antibody-generally obtained in the supernatent form (hence higher concentration is used for staining-1:100 is recommended, however standardization may help the user).

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