P-AMPK for Western Blot

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

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Company:

Cell Signaling Technologies

Product Name:

P-AMPK alpha

Catalog Number:

2535S

Clone Number:

N/A

When heavy bleeding occurs, there’s not enough blood flow to the organs in your body. Blood carries oxygen and other essential substances to your organs and tissues. When heavy bleeding occurs, these substances are lost more quickly than they can be replaced and organs in the body begin to shut down. here we check with P-AMPK alpha on Hemorrhagic shock animal model.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell and mouse tissue lysate samples

Primary Incubation

1:1000 dilution in 2% skim milk prepared in 1X TBST

Blocking Agent

2% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

Here we found the results significantly decreased P-AMPK levels.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

Antibody working Strong signals.

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