Efficient Method to Obtain a Single Cell Suspension for Heterogeneous Tissue

Osmania University
Department of Biochemistry
Research Fellow

Overall

Performance

Ease-of-Optimization

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Company:

Miltenyi Biotech

Product Name:

gentleMACS™ Dissociator

Catalog Number:

130-093-235

We have been using this instrument for isolating a single type of cells, such as macrophages and T-cells, from liver tissue. As a part of the study. we used c-type tubes from gentleMACS. Around 300 mg of liver tissue in 5 ml PBS is used to isolate kuffer cells from the liver tissue. We have used the preset protocol in macs tissue dissociater for processing the tissue. 

Experimental Design and Results Summary

Application

Tissue homogenization

Tips

Need to optimize the protocol for each tissue

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Summary

The Good

Very easy to handle, and uniform suspension of cells are obtained

The Bad

Costly consumables

The Bottom Line

If you want to isolate a single specific cell from a heterogeneous tissue. I suggest you this instrument for your workflow.

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