This BioLegend Antibody Identifies TCRb+ Cells Very Well

May 07, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 711™ anti-mouse TCR β chain Antibody

Catalog Number:

109243

Clone Number:

H57-597

We wanted to study T cells in the disease vs healthy state, so we used the antibody to identify our T cell population

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

In vitro stimulated mouse splenocyes

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody identifies the T cell population very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is great for identifying T cells in the tissue

The Bad

None to mention

The Bottom Line

The antibody clearly identifies the TCRb+ population.

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