Cell Signaling Technology
4E-BP
9452
Polyclonal
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Phosphorylation level of 4E-BP1 is detectable using this antibody based on the smear pattern.
Western Blot
wildtype HEK293T cell lysate
1:3000, 1hour at room temperature
5% non-fat milk
1:20000 anti-rabbit IRDye800
wash with TBST for 3 times, 5min each
Odyssey
Depending on the stress conditions, I can detect the change of phosphorylation on 4EBP1 protein using this antibody, although this is not a phospho-specific antibody.
N/A
High sensitivity
I often study the change of mTOR activity, and this antibody serves as a good control always.