30% Acrylamide/Bis Solution Is Great for Preparing Electrophoresis Gels for WB

March 10, 2020

UAB
Radiation Oncology
Research Scientist

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Company:

Bio-Rad

Product Name:

30% Acrylamide/Bis Solution, 29:1

Catalog Number:

1610156

Info: Supplier Page 

30% Acrylamide/Bis Solution was mixed with components to prepare electrophoresis gels for western blotting of protein lysates. The reagent can be adjusted to provide different strengths of gels depending on the gel resolution desired.

Experimental Design and Results Summary

Application

Western Blotting

Starting Material

Human breast cancer cell lines

Tips

Make sure to store the reagent at 4 degrees and be careful when handling gels with lower concentration due to less firmness

Results Summary

Gels were prepared with the acrylamide reagent and the resolutions of the bands appeared very nicely. Specific proteins with the appropriate molecular weights were detected.

DOI or PMID #

N/A

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Summary

The Good

Easy to use for gel preparation

The Bad

N/A

The Bottom Line

Acrylamide/Bis Reagent is important for providing the appropriate gel strengths required for protein detection. More Acrylamide provides greater firmness to the gel and is sufficient for detection of proteins with lower molecular weights.

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