Good Rabbit Anti-CDC25B Antibody from Proteintech

Results Summary

Graphical comparison of integrated intensity (measured by Image J) of CDC25B in germinal vesicles along the time-course assay of mouse oocyte maturation using rabbit. Error bar at each time point shows standard deviation around the mean integrated intensity of 4 different oocytes. Standard immunofluorescence (ICC) protocol was used to stain the cells with rabbit anti-CDC25B antibody (Proteintech; 1:200) and CY3-conjugated goat anti-rabbit (1:200), imaged with Olympus Fluoview FV500 confocal microscopy system. Similar results were obtained on repeating the experiment. Immunofluorescence confocal imaging reveals that CDC25B is distributed throughout the prophase I-arrested oocyte cytoplasm and some CDC25B is also present within the nucleus. During the initial stage of oocyte maturation, CDC25B accumulates in the nucleus and by 2 h after release from meiotic arrest, the concentration of CDC25B is much greater in the region where the nucleus had undergone GVBD. In all oocytes studied at each time point, the identical distribution of CDC25B was noted. Minimal background staining was observed in control oocytes processed simultaneously and imaged at the same confocal settings, but without the primary antibody.