Best Kit for the Extraction of DNA from Agarose Gel

National Dairy Research Institute
Animal Biochemistry
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Company:

Qiagen

Product Name:

QIAquick Gel Extraction Kit

Catalog Number:

28706

The PCR product of a specific gene of 2.3kb size was resolved in 1.8% agarose gel. The specific bands were visualized and clipped from the gel. The clipped agarose gel containing specific DNA band was purified using QIAquick Gel Extraction Kit. In brief, agarose gel slices were dissolved in a buffer and the mixture is applied to the QIAquick spin column. After spinning nucleic acids got adsorbed to the silica membrane of the column which was eluted in the elution buffer. The eluted DNA was used for downstream processing.

Experimental Design and Results Summary

Application

DNA purification

Starting Material

PCR product, Agarose gel, centrifuge

Tips

None

Results Summary

Upon qualitative and quantitative analysis of eluted DNA in NanoDrop, we found a high-quality DNA in a high concentration.

DOI or PMID #

None

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Summary

The Good

Can extract best quality DNA from agarose gel from even 10 μg DNA

The Bad

None

The Bottom Line

Best quality gel extraction kit.

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