Qiagen
QIAquick Gel Extraction Kit
28706
The PCR product of a specific gene of 2.3kb size was resolved in 1.8% agarose gel. The specific bands were visualized and clipped from the gel. The clipped agarose gel containing specific DNA band was purified using QIAquick Gel Extraction Kit. In brief, agarose gel slices were dissolved in a buffer and the mixture is applied to the QIAquick spin column. After spinning nucleic acids got adsorbed to the silica membrane of the column which was eluted in the elution buffer. The eluted DNA was used for downstream processing.
DNA purification
PCR product, Agarose gel, centrifuge
None
Upon qualitative and quantitative analysis of eluted DNA in NanoDrop, we found a high-quality DNA in a high concentration.
Can extract best quality DNA from agarose gel from even 10 μg DNA
Best quality gel extraction kit.