No Complaints. A Simple, Effective ELISA from BosterBio

October 25, 2019

University of Wisconsin
1415 LINDEN DRIVE
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BosterBio

Product Name:

PicoKine ELISA

Catalog Number:

EK1458

I am interested in the role of adipocyte signaling in modulating metabolic responses to diet challenges. I wanted to compare plasma FABP4 and glucose concentrations in a genetic mouse model, with impaired adipocyte signaling, under multiple dietary conditions.

Experimental Design and Results Summary

Application

Measure plasma FABP4 concentration

Starting Material

Mouse plasma (w/ EDTA as anticoagulant)

Tips

100-fold dilution of mouse plasma resulted in all data falling on standard curve.

Results Summary

Standard curve was easy to make, and 4-parameter curve fit perfect (R2>0.99). CV% for replicates on standard curve were a bit higher than I expected - around 5-10% per - but consistent with the product literature.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reagents are easy to prep or already 1X. Two vials of standard allows for breaking the kit into two assays.

The Bad

10 ml of TMB and Stop solution is cutting it close, but was not an issue. Really nothing was bad.

The Bottom Line

I'd recommend the kit. I did the assay at room temperature (instead of the recommended 37C), b/c I didn't want to deal with going back and forth into the room that houses our 37C incubator. Doing the assay at room temperature takes a bit longer (for the incubations). The data looked good (based on R2 for standard curve and CV% for replicates. In the image Columns A and B are the standard curve.

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