A Good Anti-T-Bet BioLegend Antibody

September 24, 2019

Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-T-bet Antibody

Catalog Number:

644803

Clone Number:

4B10

The aim of our research is to check the different expression profiles, in terms of cytokines and surface markers, of in vitro differentiated CD4+ T cells. This antibody was used in order to address Tbet expression in in vitro differentiated naive CD4+ T cells towards Th1 phenotype.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD4+ T cells

Primary Incubation

30mins in 4°C

Blocking Agent

PBS/BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Good, high resolution, could differentiate Th1 population among wild type and knock out cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good, sensitive, high resolution

The Bad

No bad things about it

The Bottom Line

I would recommend this antibody

Share your experience with other scientists. Write a Review! »

Join the discussion