Cell Signaling Technology
Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
3033
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The phospho-NF-kB p65 (Ser536) antibody is fantastic for detection of specific phosphorylated protein expression. Very easy to use and compatible with a variety of cancer cell lines!
Western Blot
Human breast cancer
1:1000
5% BSA in TBS-T
1:2000 Rabbit HRP
N/A
ECL with Film
Treatment of human breast cancer cell lines with chemotherapy was performed for 48 hours prior to lysis with RIPA buffer supplemented with protease/phosphatase inhibitors. Performed incubation overnight at 4 degrees. Secondary antibody at room temperature for 1 hour and developed film.
Easy to use
Use this antibody for a detection of phospho NF-kB in various human cancers with ease!