Okay Outdated Dye to Monitor Cell Proliferation

May 13, 2019

BlueskyReddit
Johns Hopkins School of Medicine
Oncology
Graduate Student

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Company:

Thermo Fisher Scientific

Product Name:

CellTrace CFSE Cell Proliferation Kit

Catalog Number:

C34554

We used this dye to monitor cell proliferation via flow cytometry of in vitro activated T cells. We were interested in assessing early proliferation within 48hrs upon activation. This dye is quite reliable for monitoring at least six to seven divisions but it has drawbacks compared to the newer dyes out there.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Naive murine T cells

Tips

Be aware of cell loss and viability issues after staining between experiments

Results Summary

We are able to monitor proliferation of naive T cells at 48 hours.

DOI or PMID #

N/A

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Summary

The Good

Able to get 7 divisions of cell division.

The Bad

Staining is not always reliable and consistent. Viability can sometimes be an issue.

The Bottom Line

Do not use CFSE. It's better to switch to other alternative proliferation dyes since they are more consistent and does not induce cell loss or viability issues.

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